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Biology/Molecular and Cellular Biology
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Gel Electrophoresis of Digested DNA

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Several DNA samples were digested with different restriction enzymes (endonucleases) and separated by gel electrophoresis, as shown.

Diagram of a vertical gel electrophoresis result with three labeled lanes at the top: EcoRI, HaeIII, and HindIII. Each lane contains horizontal black bands representing DNA fragments. The EcoRI lane h

Which of the following best explains the varying number of fragments observed in the different lanes?

A

Different running buffers were used for the different lanes.

B

There were more HindIII restriction sites than HaeIII or EcoRI restriction sites.

C

A higher voltage was applied to the first lane.

D

There were more EcoRI restriction sites than HaeIII or HindIII restriction sites.

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